Search | Engineering

订阅投稿

首页工程期刊工程焦点工程成就工程前沿关于我们 English

资源类型

期刊论文 16

年份

2023 1

2022 2

2021 2

2020 1

2017 1

2016 4

2014 2

2013 1

2011 1

2010 1

展开︾

关键词

X射线阻射性 1

miRNA簇 1

microRNA(miRNA) 1

三流体喷嘴喷雾干燥技术 1

光电神经元；神经元模型；分岔；簇放电；光电管 1

分簇算法 1

原子团簇 1

固态非晶 1

外泌体 1

多功能团簇体填料 1

多组学 1

室内定位；接收信号强度（RSS）指纹；核岭回归；簇匹配；改进型分簇 1

密度梯度 1

急冷 1

抗菌性能 1

无线自组传感器网 1

机械性能 1

毫米波通信；分簇；绕射；多路通道；传播测量 1

电子断层成像 1

展开︾

检索范围：

排序：展示方式：

促进肝癌致癌活性和细胞代谢的新型介质——miR-516a-3p

芮韬, 张学优, 冯时, 黄海涛, 詹少伟, 谢海洋, 周琳, 郑树森, 凌琪

《工程（英文）》 2022年第16卷第9期页码 162-175 doi: 10.1016/j.eng.2021.07.020

摘要：根据先前的研究，19 号染色体miRNA簇（C19MC）与肝癌患者的肿瘤高负荷和不良预后相关。目前的研究旨在探讨miR-516a-3p 在HCC 中的作用。miR-516a-3p 是一种由C19MC 4 个致癌前体miRNA（即mir-516a-1、mir-516a-2、mir-516b-1 和mir-516b-2）所共同剪接而成的相同的成熟体miRNA。

关键词：肝细胞肝癌 miRNA簇外泌体多组学

HTML PDF 收藏

Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction with

null

《医学前沿（英文）》 2014年第8卷第2期页码 217-226 doi: 10.1007/s11684-014-0326-2

摘要：

This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>10⁴ copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.

关键词： hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (HBV) mutations

HTML PDF 收藏

基于改进型分簇和核岭回归的RSS室内定位算法 Research Articles

乐燕芬,张贺娜,施伟斌,姚恒

《信息与电子工程前沿（英文）》 2021年第22卷第6期页码 827-838 doi: 10.1631/FITEE.2000093

摘要：离线阶段采用一种改进的分簇方法，采用K中心点分簇算法，把物理位置位于簇外边缘的参考点加入簇指纹库，使得参考位置点的RSS信号特性与物理位置结合。在线定位时，基于簇匹配的粗定位与簇内二次精确定位结合。簇内定位采用基于核岭回归的算法，通过核函数实现RSS信号特性与物理位置非线性关系挖掘，同时算法在簇内成员中进行，减小了时间复杂度。通过两个典型室内环境下的定位实验，探究了基于RSS信号强度和覆盖向量的两种分簇和簇匹配准则对算法性能的影响，以及不同环境下参数的选择，验证了所提算法的定位性能。

关键词：室内定位；接收信号强度（RSS）指纹；核岭回归；簇匹配；改进型分簇

HTML PDF 收藏

MiRNA-451 is a potential biomarker for estrogenicity in mouse uterus

Lingyan HOU, Yun LU, Ying LI, Li LI

《环境科学与工程前沿（英文）》 2014年第8卷第1期页码 99-105 doi: 10.1007/s11783-013-0490-7

摘要： The uterotrophic assay has been commonly used to test environmental estrogens in vivo, however, it is often not sensitive enough sometimes. An alternative way is to evaluate estrogenicity through biomarker genes. MicroRNA (miRNA) is a class of regulatory gene, which has been shown to be a good biomarker for many diseases and toxicological effects in recent years, and some evidences showed that estrogen induced response was partially mediated by miRNAs. In this study, two types of microarrays were used to test the 17β-estradiol (E2) induced miRNA expression profile at different time points in the immature mouse uterus. Statistical analysis showed the aldehyde slide based array had less variation than the amino slide based array, and 11 dysregulated miRNAs were screened out for significant fold change. Real-time PCR was performed to further confirm that 4 out of 7 selected miRNAs, namely miR-451, miR-155, miR-335-5p, and miR-365, are E2 regulated miRNAs in the uterus. The function of the predicted targets of these miRNAs is involved in cell grow control, which is consistent with the main E2 function in the uterus. MiR-451 had similar strong responses to E2 in the uterus of both immature and overiectomized mice, and could be a potential biomarker for estrogenicity in the uterus.

关键词： estrogen microRNA (miRNA) microarray biomarker

HTML PDF 收藏

基于连通可靠度约束的分簇算法研究

于继明,孙亚民,雷艳静,杨余旺

《中国工程科学》 2010年第12卷第9期页码 73-77

摘要：

在研究了一些分簇算法基础上，提出基于连通可靠度约束的、适合大规模随机部署的快速成簇算法。仿真表明基于连通可靠度约束的快速成簇算法得到的分簇覆盖面广、簇头分布合理、稳定性强，与最小ID分簇及优化的最大连接数分簇算法相比，得到簇头数量少，分簇更合理，各成员节点与簇头的连通可靠度好，能保证网络的稳定性与健壮性

关键词：无线自组传感器网连通可靠度分簇算法

HTML PDF 收藏

密集城区场景毫米波电波传播测量和分簇信道建模

张沛泽1,2,王海明1,2,洪伟1,2

《信息与电子工程前沿（英文）》 2021年第22卷第4期页码 471-487 doi: 10.1631/FITEE.2000489

摘要：考虑毫米波信道存在稀疏特性，提出改进的两步KPowerMeans多径分簇算法，在提升分簇准确性的同时有效减少了算法迭代次数。基于实测和分簇结果，可进一步分析环境因素对信道特性的影响，并建立在城市密集城区视距（LoS）和非视距（NLoS）场景下完备的多径簇统计信道模型。信道分簇统计建模结果表明：在密集城区场景通常存在5–8个多径散射簇，具体的簇数目与环境密切相关，多径主要由建筑物外表面的一次和二次反射及屋顶和侧边绕射引起；由于存在丰富的多径，在适当的系统配置下通过架高发射天线，毫米波系统在NLoS场景下的覆盖范围可达到200米以上；相比于全向信道而言，簇内时延和水平角度扩展远小于全向信道的结果，并且随着工作频率升高，两者均呈现减小趋势；当接收机或用户在同一水平维度时，全向和簇内接收俯仰角度扩展近似综上，基于大量的信道实测数据及信道分簇结果，揭示了环境散射体与多径散射簇的映射关系，文中给出反射和绕射等传播机制对信道参数的影响，并在典型频段构建了适用于城市密集城区场景的三维信道统计模型，建模结果有效弥补了

关键词：毫米波通信；分簇；绕射；多路通道；传播测量

HTML PDF 收藏

Inflammation and liver tumorigenesis

null

《医学前沿（英文）》 2013年第7卷第2期页码 242-254 doi: 10.1007/s11684-013-0256-4

摘要：

Inflammation has been considered as one of the hallmarks of cancer, and chronic hepatitis is a major cause of liver cancer. This review will focus on the pathogenic role of inflammation in hepatocarcinogenesis and will discuss recent advances in understanding the chronic hepatitis-liver cancer link based on hot spots in liver cancer research, including cellular interaction, cytokines, microRNA and stem cells. All of these mechanisms should be taken into consideration because they are crucial for the development of more efficacious therapeutic strategies for preventing and treating human chronic hepatitis and hepatocellular carcinoma.

关键词： hepatocellular carcinoma hepatitis cytokine stem cell miRNA

HTML PDF 收藏

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ

《环境科学与工程前沿（英文）》 2016年第10卷第3期页码 513-521 doi: 10.1007/s11783-015-0777-y

摘要： The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production using anaerobic granular sludge as inoculum and, secondly, to study the metabolic shifts of an anaerobic community subjected to low partial gas pressures. With this goal in mind, seven different wastewater (four synthetic media, two industrial wastewater, and one domestic effluent) and the effect of applying vacuum on the systems were analyzed. The application of vacuum promoted an increase in the diversity of hydrogen-producing bacteria, such as , and promoted the dominance of acetoclastic- over hydrogenotrophic methanogens. The application of different media promoted a wide variety of metabolic pathways. Nevertheless, reduction of the hydrogen partial pressure by application of vacuum lead to further oxidation of reaction intermediates irrespective of the medium used, which resulted in higher hydrogen and methane production, and improved the COD removal. Interestingly, vacuum greatly promoted biogenic hydrogen production from a real wastewater, which opens possibilities for future application of dark fermentation systems to enhance biohydrogen yields.

关键词： dark fermentation biohydrogen wastewaters vacuum

HTML PDF 收藏

miRNAs in non-alcoholic fatty liver disease

null

《医学前沿（英文）》 2016年第10卷第4期页码 389-396 doi: 10.1007/s11684-016-0468-5

摘要：

Non-alcoholic fatty liver disease (NAFLD) is a major cause of liver cirrhosis and hepatocellular carcinoma and is a considerable threat to public health. miRNAs are important post-transcriptional regulators of gene expression, and the dysregulation of miRNAs is involved in various biological processes in the liver, including lipid homeostasis, inflammation, apoptosis, and cell proliferation. Recently, a number of studies have described the association between miRNAs and NAFLD progression and have shown that circulating miRNAs reflect histological changes in the liver. Therefore, circulating miRNAs have potential use for the evaluation of NAFLD severity. In this review, we discuss the involvement of miRNAs in NAFLD pathogenesis and the key role of miRNAs in the screening, diagnosis, and staging of NAFLD.

关键词： nonalcoholic fatty liver disease nonalcoholic steatohepatitis hepatocellular carcinoma miRNA

HTML PDF 收藏

MicroRNAs and their roles in osteoclast differentiation

Zhuying Xia, Chao Chen, Peng Chen, Hui Xie, Xianghang Luo

《医学前沿（英文）》 2011年第5卷第4期页码 414-419 doi: 10.1007/s11684-011-0168-0

摘要： Osteoclasts, which are derived from monocyte-macrophage precursors, are exclusive bone resorption cells. Limited evidence indicates that a class of small non-coding single-stranded RNAs known as “microRNAs” (miRNAs) is also involved in bone resorption. Although various miRNAs regulate cell proliferation and differentiation, few miRNAs have been reported to play a key role in the regulation of osteoclast differentiation. In this short review, the biology and functional mechanisms of miRNAs in osteoclastogenesis are summarized. The profiling, function, and target prediction of miRNAs are discussed as well.

关键词： miRNA osteoclast differentiation antagomirs

HTML PDF 收藏

一种采用三流体喷嘴喷雾干燥技术高效构筑齿科修复用多功能团簇体填料的通用策略 Article

杨丹蕾, 王丹, 牛昊, 王瑞莉, 刘梅, 章非敏, 王洁欣, 朱美芳

《工程（英文）》 2022年第8卷第1期页码 138-147 doi: 10.1016/j.eng.2021.08.001

摘要：本研究首次采用配备三流体喷嘴的喷雾干燥设备构筑由不同功能纳米颗粒组成的高性能复合纳米颗粒团簇体，并将其作为填料应用于齿科修复。三流体喷嘴的应用可以有效避免喷雾干燥前需将带有相反电荷的纳米粒子混合而发生的团聚现象，从而构筑形貌规则的复合纳米颗粒团簇体填料。此外，对SiO₂-ZrO2复合团簇体填料进行热处理后再进行填充，能够显著提高复合树脂的力学性能和X射线阻射性。含有10 wt.填充了SiO₂-ZrO₂-ZnO 复合团簇体填料（三者质量比为56∶10∶4）的复合树脂，除了具有良好的力学性能和X射线阻射性外，还展现出高的抗菌活性（抗菌率因此，采用三流体喷嘴的喷雾干燥技术在高效构筑多功能团簇体填料方面具有巨大的潜力。

关键词：多功能团簇体填料三流体喷嘴喷雾干燥技术机械性能抗菌性能 X射线阻射性齿科复合树脂

HTML PDF 收藏

U-shaped microRNA expression pattern could be a new concept biomarker for environmental estrogen

Rui Duan, Yun Lu, Lingyan Hou, Lina Du, Lequn Sun, Xingfan Tang

《环境科学与工程前沿（英文）》 2016年第10卷第6期 doi: 10.1007/s11783-016-0880-8

摘要： Estrogen regulates miRNA expression in a typical U-shaped dose-response pattern. E2 can regulate in the ventral prostate. Mouse ventral prostate is most sensitive to estrogen. Nonmonotonic dose-response in prostate could be a component of estrogen signature. Many studies have focused on environmental estrogen-related diseases. However, no consistent gene markers or signatures for estrogenicity have been discovered in mammals. This study investigated the estrogenic effects of 17β-estradiol on the prostate in immature male mice. Consistent U-shaped responses were seen in bodyweight, ventral prostate epithelial morphology, and miRNA expression levels. Specifically, most estradiol regulated miRNAs were downregulated at low doses of estradiol (0.2 and 2 mg·kg ), and whose expression returned to the control level at a larger dose (200 mg·kg ). The function of these regulated miRNAs is related to the prostate cancer and PI3K-Akt signaling pathways, which is consistent with the function of estradiol. Furthermore, the miRNA-processing machinery, Drosha, in the prostate was also regulated in a similar pattern, which could be a part of the U-shaped miRNA expression mechanism. All of these data indicate that the prostate is a reliable organ for evaluating estrogenic activity and that the typical nonmonotonic dose-response relationship could be used as a novel biomarker for estrogenicity.

关键词： miRNA Prostate Estradiol Nonmonotonic dose-response Estrogenicity Drosha

HTML PDF 收藏

Global transcriptome analysis for identification of interactions between coding and noncoding RNAs during human erythroid differentiation

null

《医学前沿（英文）》 2016年第10卷第3期页码 297-310 doi: 10.1007/s11684-016-0452-0

摘要：

Studies on coding genes, miRNAs, and lncRNAs during erythroid development have been performed in recent years. However, analysis focusing on the integration of the three RNA types has yet to be done. In the present study, we compared the dynamics of coding genes, miRNA, and lncRNA expression profiles. To explore dynamic changes in erythropoiesis and potential mechanisms that control these changes in the transcriptome level, we took advantage of high throughput sequencing technologies to obtain transcriptome data from cord blood hematopoietic stem cells and the following four erythroid differentiation stages, as well as from mature red blood cells. Results indicated that lncRNAs were promising cell marker candidates for erythroid differentiation. Clustering analysis classified the differentially expressed genes into four subtypes that corresponded to dynamic changes during stemness maintenance, mid-differentiation, and maturation. Integrated analysis revealed that noncoding RNAs potentially participated in controlling blood cell maturation, and especially associated with heme metabolism and responses to oxygen species and DNA damage. These regulatory interactions were displayed in a comprehensive network, thereby inferring correlations between RNAs and their associated functions. These data provided a substantial resource for the study of normal erythropoiesis, which will permit further investigation and understanding of erythroid development and acquired erythroid disorders.

关键词： erythroid differentiation hematopoietic stem cell RNA-seq miRNA lncRNA

HTML PDF 收藏

金属玻璃形成过程中纳米密度梯度对结构的稳定作用 Article

周少雄, 董帮少, 王岩国, 秦敬玉, 汪卫华

《工程（英文）》 2023年第29卷第10期页码 120-129 doi: 10.1016/j.eng.2023.01.010

摘要：本文利用电子断层成像和基于非晶模型的成像模拟技术表征了Zr60Cu30Al10金属玻璃中的纳米密度梯度结构，该纳米密度梯度源于按原子堆垛密度递减顺序排列的原子团簇群，并构成了不均匀的金属玻璃中程微观结构三维重构像中由特定表面构成的大尺寸多面体与类二十面体团簇对应，同时也揭示了这些高原子堆垛密度原子团簇的空间分布情况。各种原子团簇按原子堆垛密度递减方式排列作为合金熔体玻璃化凝固过程中潜在的本征分布规律，能够起到稳定过冷熔体和金属玻璃结构的作用。

关键词：急冷固态非晶密度梯度电子断层成像原子团簇